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验证数据展示

  • IHC staining of human liver using 23849-1-AP

    IHC staining of human liver using 23849-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23849-1-AP (IER2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IER2 antibody included
  • IHC staining of human liver using 23849-1-AP

    IHC staining of human liver using 23849-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23849-1-AP (IER2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IER2 antibody included

IER2 Polyclonal antibody

IER2 Polyclonal Antibody for IHC, ELISA
Cat No. 23849-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA and More (1)

别名

3CH92, CHX1, ETR101, IER2, immediate early response 2, Pip92, Protein ETR101

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IER2 Ready-To-Use IHC Kit. IER2 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

23849-1-AP targets IER2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 IER2 fusion protein Ag20869 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 immediate early response 2
别名 3CH92, CHX1, ETR101, IER2, immediate early response 2, Pip92, Protein ETR101
计算分子量 223 aa, 24 kDa
GenBank蛋白编号BC072432
基因名称 IER2
Gene ID (NCBI) 9592
RRIDAB_2879339
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9BTL4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ier2 (also called ETR101, Pip92, 3CH92 and CHX1, hereafter referred to as Ier2, regardless of species) is an immediate early gene (PMID: 2678945) that was originally found to be upregulated in fibroblasts upon their activation (PMID: 3841511). Immediate early genes are a group of genes that are rapidly induced in quiescent cells by proliferation- and migration-inducing stimuli. Ier2 may have a role in tumor cell motility and metastasis (PMID: 22120713).

实验方案

Product Specific Protocols
IHC protocol for IER2 antibody 23849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Signal

IER family proteins are regulators of protein phosphatase PP2A and modulate the phosphorylation status of CDC25A.

Authors - Takumi Ueda
View Article
humanWB

FEBS J

PP2A-B55 and its adapter proteins IER2 and IER5 regulate the activity of RB family proteins and the expression of cell cycle-related genes

Authors - Kuriko Doi
View Article