验证数据展示
经过测试的应用
Positive WB detected in | mouse testis tissue |
Positive IHC detected in | human breast cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | DU 145 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 5 publications below |
产品信息
20979-1-AP targets IKBKB in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IF, IHC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta |
Synonyms | I kappa B kinase 2, I kappa B kinase beta, IKBKB, IKK B, IKK beta, IKK2, IKKB, IκB, NFKBIKB |
Calculated Molecular Weight | 87 kDa |
Observed Molecular Weight | 80 kDa, 86 kDa, 87 kDa |
GenBank Accession Number | NM_001556 |
Gene Symbol | IKBKB |
Gene ID (NCBI) | 3551 |
RRID | AB_2878780 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O14920 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IKBKB, also named as NFKBIKB, IKK2 and IKKB, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and I-kappa-B kinase subfamily. IKBKB acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. It also phosphorylates NCOA3. The antibody is specific to IKBKB. This antibody can identify 3 isoform of IKBKB with the molecular weight of 80, 86, 87 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for IKBKB antibody 20979-1-AP | Download protocol |
IHC protocol for IKBKB antibody 20979-1-AP | Download protocol |
IF protocol for IKBKB antibody 20979-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Redox Biol Cardioprotection of CAPE-oNO2 against myocardial ischemia/reperfusion induced ROS generation via regulating the SIRT1/eNOS/NF-κB pathway in vivo and in vitro. | ||
Neurochem Res Intrathecal Delivery of ssAAV9-DAO Extends Survival in SOD1(G93A) ALS Mice. |