验证数据展示
经过测试的应用
Positive IHC detected in | human tonsillitis tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 2 publications below |
ELISA | See 1 publications below |
产品信息
22859-1-AP targets IL-31 in IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | IHC, ELISA Application Description |
Cited Applications | IHC, ELISA |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | IL-31 fusion protein Ag18848 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | interleukin 31 |
Synonyms | IL 31, IL31, IL-31, interleukin 31 |
Calculated Molecular Weight | 164 aa, 18 kDa |
GenBank Accession Number | BC132998 |
Gene Symbol | IL-31 |
Gene ID (NCBI) | 386653 |
RRID | AB_2879179 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q6EBC2 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IL-31 mRNA encodes a 164-aa precursor and a predicted 141-aa mature polypeptide, displaying an apparent molecular mass of 24 kDa in its glycosylated form. IL31 is a newly discovered four-helix bundle cytokine and expressed by several types of cells under both normal and disease state, such as activated CD4+T cells, mast cells, monocytes, macrophages, immature and mature monocyte-derived dendritic cells and so on. IL-31 signals through a heterodimeric receptor complex consisting of the IL31 receptor alpha (IL31Rα) and the oncostatin M receptor (OSMR). IL-31 plays a predominant function in the mediation of inflammatory and lymphoma-associated itch. Increased IL-31 expression has been detected in inflamed tissues from patients with atopic dermatitis, bowel diseases, allergic asthma and rhinitis.
实验方案
Product Specific Protocols | |
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IHC protocol for IL-31 antibody 22859-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
World J Gastrointest Oncol Clinical implications of interleukins-31, 32, and 33 in gastric cancer |