CD126/IL-6R alpha Monoclonal antibody
CD126/IL-6R alpha Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2A5F4
验证数据展示
经过测试的应用
Positive WB detected in | human spleen tissue, Human peripheral blood leukocyte |
Positive IHC detected in | human liver cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
66855-1-Ig targets CD126/IL-6R alpha in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | IF |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | CD126/IL-6R alpha fusion protein Ag18263 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | interleukin 6 receptor |
Synonyms | CD126, IL-6R alpha, 2A5F4, IL 6R alpha, IL 6R 1 |
Calculated Molecular Weight | 468 aa, 52 kDa |
Observed Molecular Weight | 80 kDa |
GenBank Accession Number | BC132684 |
Gene Symbol | IL-6R alpha |
Gene ID (NCBI) | 3570 |
RRID | AB_2882195 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P08887 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CD126/IL-6R alpha antibody 66855-1-Ig | Download protocol |
IHC protocol for CD126/IL-6R alpha antibody 66855-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Immunol Expression and clinical significance of interleukin-6 pathway in cholangiocarcinoma |