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验证数据展示

  • WB analysis of L02 using 21540-1-AP

    WB analysis of L02 using 21540-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21540-1-AP (ISM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 21540-1-AP

    IHC staining of human placenta using 21540-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21540-1-AP (ISM2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 21540-1-AP

    IHC staining of human placenta using 21540-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21540-1-AP (ISM2 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of L02 using 21540-1-AP

    IF Staining of L02 using 21540-1-AP

    Immunofluorescent analysis of L02 cells using 21540-1-AP (ISM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ISM2 Polyclonal antibody

ISM2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21540-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Thrombospondin and AMOP domain-containing isthmin-like protein 1, TAIL1, PSEC0137, Isthmin-2, isthmin 2 homolog (zebrafish)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, mouse kidney tissue, mouse pancreas tissue, mouse skin tissue
Positive IHC detected inhuman placenta tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21540-1-AP targets ISM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 ISM2 fusion protein Ag16187 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 isthmin 2 homolog (zebrafish)
别名 Thrombospondin and AMOP domain-containing isthmin-like protein 1, TAIL1, PSEC0137, Isthmin-2, isthmin 2 homolog (zebrafish)
计算分子量 571 aa, 64 kDa
观测分子量 65 kDa, 55 kDa
GenBank蛋白编号BC101020
基因名称 ISM2
Gene ID (NCBI) 145501
RRIDAB_2878880
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6H9L7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ISM2 antibody 21540-1-APDownload protocol
IHC protocol for ISM2 antibody 21540-1-APDownload protocol
IF protocol for ISM2 antibody 21540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols