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CD11B/Integrin Alpha M Polyclonal antibody

CD11B/Integrin Alpha M Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 21851-1-AP

Print datasheet

Synonyms

CD11b, CD11 antigen-like family member B, CR3A, CR-3 alpha chain, CR 3 alpha chain



经过测试的应用

Positive WB detected inTF-1 cells
Positive IHC detected inhuman tonsillitis tissue, Insulinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells
Planning an IHC experiment? We recommend our IHCeasy CD11B Ready-To-Use IHC Kit. CD11B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21851-1-AP targets CD11B/Integrin Alpha M in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman, pig, sheep
Immunogen CD11B/Integrin Alpha M fusion protein Ag16327 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Synonyms CD11b, CD11 antigen-like family member B, CR3A, CR-3 alpha chain, CR 3 alpha chain
Calculated Molecular Weight 1152 aa, 127 kDa
Observed Molecular Weight170 kDa
GenBank Accession NumberBC096346
Gene Symbol CD11b
Gene ID (NCBI) 3684
ENSEMBL Gene IDENSG00000169896
RRIDAB_2878927
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11215
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

实验方案

Product Specific Protocols
WB protocol for CD11B/Integrin Alpha M antibody 21851-1-APDownload protocol
IHC protocol for CD11B/Integrin Alpha M antibody 21851-1-APDownload protocol
IF protocol for CD11B/Integrin Alpha M antibody 21851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,WB

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FC

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ACS Appl Mater Interfaces

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Int J Biol Macromol

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IF

Biomater Sci

A hybrid hydrogel composed of chitin and β-glucan for the effective management of wound healing and scarring

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humanWB

Int J Mol Sci

Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.

Authors - Krishnendu Khan