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验证数据展示

  • WB analysis of PC-3 using 12300-1-AP

    WB analysis of PC-3 using 12300-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse thymus using 12300-1-AP

    IP experiment of mouse thymus using 12300-1-AP

    IP result of anti-ITGB1BP1 (IP:12300-1-AP, 4ug; Detection:12300-1-AP 1:300) with mouse thymus tissue lysate 2800ug.

  • IHC staining of human heart using 12300-1-AP

    IHC staining of human heart using 12300-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12300-1-AP (ITGB1BP1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 12300-1-AP

    IHC staining of human heart using 12300-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12300-1-AP (ITGB1BP1 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of PC-3 using 12300-1-AP

    IF Staining of PC-3 using 12300-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ITGB1BP1 antibody (12300-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ITGB1BP1 Polyclonal antibody

ITGB1BP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12300-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Integrin cytoplasmic domain-associated protein 1, Integrin beta-1-binding protein 1, ICAP-1, ICAP1, ICAP 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, mouse thymus tissue, human brain tissue
Positive IP detected inmouse thymus tissue
Positive IHC detected inhuman heart tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12300-1-AP targets ITGB1BP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ITGB1BP1 fusion protein Ag2945 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrin beta 1 binding protein 1
Synonyms Integrin cytoplasmic domain-associated protein 1, Integrin beta-1-binding protein 1, ICAP-1, ICAP1, ICAP 1
Calculated Molecular Weight 200 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC012264
Gene Symbol ITGB1BP1
Gene ID (NCBI) 9270
RRIDAB_10640896
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14713
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ITGB1BP1, also named as ICAP1, is a key regulator of the integrin-mediated cell-matrix interaction signaling by binding to the ITGB1 cytoplasmic tail. It plays a role in cell proliferation, differentiation, spreading, adhesion and migration in the context of mineralization and bone development and angiogenesis. ITGB1BP1 promotes transcriptional activity of the MYC promoter.

实验方案

Product Specific Protocols
WB protocol for ITGB1BP1 antibody 12300-1-APDownload protocol
IHC protocol for ITGB1BP1 antibody 12300-1-APDownload protocol
IF protocol for ITGB1BP1 antibody 12300-1-APDownload protocol
IP protocol for ITGB1BP1 antibody 12300-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols