验证数据展示
经过测试的应用
Positive WB detected in | PC-3 cells, mouse thymus tissue, human brain tissue |
Positive IP detected in | mouse thymus tissue |
Positive IHC detected in | human heart tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | PC-3 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12300-1-AP targets ITGB1BP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | ITGB1BP1 fusion protein Ag2945 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | integrin beta 1 binding protein 1 |
Synonyms | Integrin cytoplasmic domain-associated protein 1, Integrin beta-1-binding protein 1, ICAP-1, ICAP1, ICAP 1 |
Calculated Molecular Weight | 200 aa, 22 kDa |
Observed Molecular Weight | 22 kDa |
GenBank Accession Number | BC012264 |
Gene Symbol | ITGB1BP1 |
Gene ID (NCBI) | 9270 |
RRID | AB_10640896 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O14713 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ITGB1BP1, also named as ICAP1, is a key regulator of the integrin-mediated cell-matrix interaction signaling by binding to the ITGB1 cytoplasmic tail. It plays a role in cell proliferation, differentiation, spreading, adhesion and migration in the context of mineralization and bone development and angiogenesis. ITGB1BP1 promotes transcriptional activity of the MYC promoter.
实验方案
Product Specific Protocols | |
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WB protocol for ITGB1BP1 antibody 12300-1-AP | Download protocol |
IHC protocol for ITGB1BP1 antibody 12300-1-AP | Download protocol |
IF protocol for ITGB1BP1 antibody 12300-1-AP | Download protocol |
IP protocol for ITGB1BP1 antibody 12300-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |