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  • Featured Product
  • KD/KO Validated

Cytokeratin 13 Monoclonal antibody

Cytokeratin 13 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1E10G2

Cat no : 66684-1-Ig

Print datasheet

Synonyms

KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13

验证数据展示

  • WB analysis of A431 using 66684-1-Ig

    WB analysis of A431 using 66684-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 66684-1-Ig (KRT13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 66684-1-Ig

    IHC staining of human cervical cancer using 66684-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (KRT13 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT13 antibody included
  • IHC staining of human cervical cancer using 66684-1-Ig

    IHC staining of human cervical cancer using 66684-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (KRT13 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT13 antibody included
  • IF Staining of human cervical cancer using 66684-1-Ig

    IF Staining of human cervical cancer using 66684-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using Cytokeratin 13 antibody (66684-1-Ig, Clone: 1E10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of A431 using 66684-1-Ig

    WB analysis of A431 using 66684-1-Ig

    WB result of Cytokeratin 13 antibody (66684-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 13 transfected A431 cells.

经过测试的应用

Positive WB detected inA431 cells, rat skin tissue
Positive IHC detected inhuman cervical cancer tissue, human oesophagus cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman cervical cancer tissue
Planning an IHC experiment? We recommend our IHCeasy KRT13 Ready-To-Use IHC Kit. KRT13 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66684-1-Ig targets Cytokeratin 13 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, rat
Immunogen Cytokeratin 13 fusion protein Ag0217 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name keratin 13
Synonyms KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC002661
Gene Symbol Cytokeratin 13
Gene ID (NCBI) 3860
RRIDAB_2882038
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13646
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. The Keratin 13 (KRT13) gene encodes a type I acidic keratin which is expressed in the differentiated cells of non-cornified stratified squamous epithelia. This antibody can react with Cytokeratin 15.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 13 antibody 66684-1-IgDownload protocol
IHC protocol for Cytokeratin 13 antibody 66684-1-IgDownload protocol
IF protocol for Cytokeratin 13 antibody 66684-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols