验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, Jurkat cells, K-562 cells, PC-3 cells, SKOV-3 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human ovary tumor tissue, human bowen disease, human colon tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy KTN1 Ready-To-Use IHC Kit. KTN1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19841-1-AP targets KTN1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, CoIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, monkey |
| Immunogen | KTN1 fusion protein Ag13854 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | kinectin 1 (kinesin receptor) |
| Synonyms | KTN 1, KIAA0004, CG-1 antigen |
| Calculated Molecular Weight | 1357 aa, 156 kDa |
| Observed Molecular Weight | 156 kDa |
| GenBank Accession Number | BC117132 |
| Gene Symbol | KTN1 |
| Gene ID (NCBI) | 3895 |
| RRID | AB_10642436 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86UP2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KTN1/Kinectin is an integral endoplasmic reticulum (ER) membrane protein which acts as dynamic anchor to facilitate ER membrane extension along microtubules. It has been found to bind kinesin, Rho GTPase, and translation elongation factor-1delta and gets involved in organelle motility and protein synthesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KTN1 antibody 19841-1-AP | Download protocol |
| IHC protocol for KTN1 antibody 19841-1-AP | Download protocol |
| IF protocol for KTN1 antibody 19841-1-AP | Download protocol |
| IP protocol for KTN1 antibody 19841-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature ER proteins decipher the tubulin code to regulate organelle distribution
| ||
Sci Transl Med CPEB alteration and aberrant transcriptome-polyadenylation lead to a treatable SLC19A3 deficiency in Huntington's disease. | ||
Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. | ||
EMBO Rep Mobile late endosomes modulate peripheral endoplasmic reticulum network architecture. | ||
Clin Sci (Lond) The long noncoding RNA KTN1-AS1 promotes bladder cancer tumorigenesis via KTN1 cis-activation and the consequent initiation of Rho GTPase-mediated signaling. | ||
