验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, rat brain tissue, mouse small intestine tissue, SGC-7901 cells, MCF-7 cells |
| Positive IHC detected in | human liver cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15707-1-AP targets L2HGDH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | L2HGDH fusion protein Ag8382 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | L-2-hydroxyglutarate dehydrogenase |
| 别名 | C14orf160, DURANIN, L2HGDH |
| 计算分子量 | 463aa,50 kDa; 441aa,49 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC006117 |
| 基因名称 | L2HGDH |
| Gene ID (NCBI) | 79944 |
| RRID | AB_2133202 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H9P8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
L2HGDH(L-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as duranin, C14orf160 and belongs to the L2HGDH family. The putative L2HGDH is predicted to be targeted to the mitochondria where its mitochondrial targeting sequence is presumably removed(PMID:16005139). Defects in L2HGDH are the cause of L-2-hydroxyglutaric aciduria (L2HGA). It has 2 isoforms produced by alternative splicing with the molecular weight of 50 kDa and 48 kDa. L2HGDH also can be detected as ~45kD due to the 51aa transit peptide cleaved.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for L2HGDH antibody 15707-1-AP | Download protocol |
| IHC protocol for L2HGDH antibody 15707-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Hypoxia-Mediated Increases in L-2-hydroxyglutarate Coordinate the Metabolic Response to Reductive Stress.
| ||
Sci Transl Med 2-Hydroxyglutarate produced by neomorphic IDH mutations suppresses homologous recombination and induces PARP inhibitor sensitivity.
| ||
Cancer Discov L-2-Hydroxyglutarate: an epigenetic modifier and putative oncometabolite in renal cancer. | ||
Cell Chem Biol MYC Regulation of D2HGDH and L2HGDH Influences the Epigenome and Epitranscriptome.
| ||
Clin Cancer Res Biochemical and Epigenetic Insights into L-2-Hydroxyglutarate, a Potential Therapeutic Target in Renal Cancer. |
