LGR5 Polyclonal antibody

LGR5 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 21833-1-AP

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Synonyms

FEX, G protein coupled receptor 49, G protein coupled receptor 67, GPR49, GPR67, GRP49, HG38, LGR5



经过测试的应用

Positive IHC detected inhuman small intestine tissue, human colon tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21833-1-AP is LGR5 Fusion Protein expressed in E. coli.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen LGR5 fusion protein Ag16389 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine-rich repeat-containing G protein-coupled receptor 5
Synonyms FEX, G protein coupled receptor 49, G protein coupled receptor 67, GPR49, GPR67, GRP49, HG38, LGR5
Calculated Molecular Weight 907 aa, 100 kDa
GenBank Accession NumberBC096324
Gene Symbol LGR5
Gene ID (NCBI) 8549
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75473
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LGR5, also named as GPR49, GPR67 and HG38, belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. LGR5 is a stem cell marker of the intestinal epithelium and the hair follicule. It is a target gene of Wnt signaling.

发表文章

SpeciesApplicationTitle
humanIHC

Oncotarget

LGR5 regulates pro-survival MEK/ERK and proliferative Wnt/β-catenin signalling in neuroblastoma.

Authors - Gabriella Cunha Vieira
humanIF

Inflamm Bowel Dis

Overexpression of Cancer-Associated Stem Cell Gene OLFM4 in the Colonic Epithelium of Patients With Primary Sclerosing Cholangitis.

Authors - Mastura Neyazi
mouseWB

J Dermatol Sci

REGγ regulates hair cycle by activating Lgr5 positive hair follicle stem cells.

Authors - Xiao Gao

Tumour Biol

Correlation of Musashi-1, Lgr5, and pEGFR expressions in human small intestinal adenocarcinomas.

Authors - Yan Wang

Oncol Lett

Detection of circulating colorectal cancer cells by a custom microfluid system before and after endoscopic metallic stent placement.

Authors - Rei Ishibashi
humanIHC

Oncol Lett

Expression and clinical significance of SALL4 and LGR5 in patients with lung cancer.

Authors - Ajay Kumar Gautam