验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, K-562 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | NCCIT cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24017-1-AP targets LIN28B in WB, IHC, IF/ICC, IP, ELISA, PLA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, PLA |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | LIN28B fusion protein Ag21241 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | lin-28 homolog B (C. elegans) |
| Synonyms | Protein lin 28 homolog B, Lin-28B, Lin28, Lin 28B, lin 28 homolog B (C. elegans) |
| Calculated Molecular Weight | 250 aa, 27 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC137526 |
| Gene Symbol | LIN28B |
| Gene ID (NCBI) | 389421 |
| RRID | AB_2879403 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZN17 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LIN28B, also named as CSDD2, belongs to the lin-28 family. It acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7). LIN2B is specifically expressed in embryonic muscle, neurons, and epithelia, as well as in embryonic carcinoma cells. However, it is suppressed in most adult tissues except for skeletal and cardiac muscle. The calculated molecular weight of LIN28B is 27 kDa, but a 35 kDa band has been detected in some articles (PMID: 33029224, 33378003).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LIN28B antibody 24017-1-AP | Download protocol |
| IF protocol for LIN28B antibody 24017-1-AP | Download protocol |
| IP protocol for LIN28B antibody 24017-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Immunol Lin28 regulates thymic growth and involution and correlates with MHCII expression in thymic epithelial cells | ||
iScience Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells | ||
Pharm Biol Peptides from the croceine croaker (Larimichthys crocea) swim bladder attenuate busulfan-induced oligoasthenospermia in mice. | ||
Cell Biol Toxicol The pivotal role of ZNF384: driving the malignant behavior of serous ovarian cancer cells via the LIN28B/UBD axis |