LYN Polyclonal antibody
LYN Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HL-60 cells, Jurkat cells, K-562 cells, L02 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy LYN Ready-To-Use IHC Kit. LYN primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18135-1-AP targets LYN in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | LYN fusion protein Ag12723 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | v-yes-1 Yamaguchi sarcoma viral related oncogene homolog |
| Synonyms | V-yes-1 Yamaguchi sarcoma viral related oncogene homolog, Tyrosine-protein kinase Lyn, Tyrosine protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase, JTK8 |
| Calculated Molecular Weight | 512 aa, 59 kDa |
| Observed Molecular Weight | 53-56 kDa, 66-70 kDa |
| GenBank Accession Number | BC075002 |
| Gene Symbol | LYN |
| Gene ID (NCBI) | 4067 |
| RRID | AB_11042319 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07948 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LYN antibody 18135-1-AP | Download protocol |
| IHC protocol for LYN antibody 18135-1-AP | Download protocol |
| IF protocol for LYN antibody 18135-1-AP | Download protocol |
| IP protocol for LYN antibody 18135-1-AP | Download protocol |
| FC protocol for LYN antibody 18135-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Invest Dermatol Loss of LYN is frequent in targeted-therapy resistant melanoma cells and favors metastatic properties
| ||
