验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, human brain tissue, Raji cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells, SH-SY5Y cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IF | See 1 publications below |
产品信息
18322-1-AP targets MAD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | MAD1 fusion protein Ag13171 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | MAD1 mitotic arrest deficient-like 1 (yeast) |
| Synonyms | MAD1L1, Tax-binding protein 181, Mitotic arrest deficient 1-like protein 1, MAD1-like protein 1, HsMAD1 |
| Calculated Molecular Weight | 83 kDa |
| Observed Molecular Weight | 83 kDa |
| GenBank Accession Number | BC009964 |
| Gene Symbol | MAD1 |
| Gene ID (NCBI) | 8379 |
| RRID | AB_2139251 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6D9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAD1L1 is a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. It recruits MAD2L1 to unattached kinetochores. Also it is required for anchoring MAD2L1 to the nuclear periphery. In addition, it binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding [PMID:10049595, 12837246].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAD1 antibody 18322-1-AP | Download protocol |
| IHC protocol for MAD1 antibody 18322-1-AP | Download protocol |
| IF protocol for MAD1 antibody 18322-1-AP | Download protocol |
| IP protocol for MAD1 antibody 18322-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Cycle Nuclear interactor of ARF and Mdm2 regulates multiple pathways to activate p53. | ||
BMB Rep TCP10L synergizes with MAD1 in transcriptional suppression and cell cycle arrest through mutual interaction. | ||
Sci Adv Biallelic germline mutations in MAD1L1 induce a syndrome of aneuploidy with high tumor susceptibility | ||
Aging (Albany NY) The HNF4A-CHPF pathway promotes proliferation and invasion through interactions with MAD1L1 in glioma |
