验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, Raji cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy MCM3 Ready-To-Use IHC Kit. MCM3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15597-1-AP targets MCM3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | MCM3 fusion protein Ag7966 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | minichromosome maintenance complex component 3 |
| 别名 | P1-MCM3, p102, P1 MCM3, EC:3.6.4.12, DNA polymerase alpha holoenzyme-associated protein P1 |
| 计算分子量 | 91 kDa |
| 观测分子量 | 102-105 kDa |
| GenBank蛋白编号 | BC001626 |
| 基因名称 | MCM3 |
| Gene ID (NCBI) | 4172 |
| RRID | AB_2141973 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P25205 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MCM3, also named as HCC5, P1-MCM3, p102, RLF subunit beta and RLFB, belongs to the MCM family. MCM3 is essential for the initiation of DNA replication and is involved in ensuring that DNA replication is initiated precisely once per cell cycle(PMID: 33093917). MCM3 has 2 isoforms with the molecular mass of 91 and 96 kDa. The overexpression of MCM3 is related to proliferation of tumor cells(PMID: 31186514).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MCM3 antibody 15597-1-AP | Download protocol |
| IHC protocol for MCM3 antibody 15597-1-AP | Download protocol |
| IP protocol for MCM3 antibody 15597-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer. | ||
Biomaterials Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis. | ||
Aging Cell Pyrroloquinoline quinone alleviates natural aging-related osteoporosis via a novel MCM3-Keap1-Nrf2 axis-mediated stress response and Fbn1 upregulation
| ||
Elife MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells. | ||
Oncotarget Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis. | ||
Ecotoxicol Environ Saf The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis. |
