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MEIS2 Polyclonal antibody

MEIS2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11550-1-AP

Print datasheet

Synonyms

Homeobox protein Meis2, HsT18361, Meis homeobox 2, Meis1 related protein 1, MEIS2, MRG1

验证数据展示

  • WB analysis using 11550-1-AP

    WB analysis using 11550-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11550-1-AP (MEIS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 11550-1-AP

    WB analysis using 11550-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11550-1-AP (MEIS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 11550-1-AP

    IHC staining of human prostate cancer using 11550-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11550-1-AP (MEIS2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 11550-1-AP

    IHC staining of human prostate cancer using 11550-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11550-1-AP (MEIS2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, PC-3 cells, A549 cells, LNCaP cells, HCT 116 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

11550-1-AP targets MEIS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen MEIS2 fusion protein Ag2096 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Meis homeobox 2
Synonyms Homeobox protein Meis2, HsT18361, Meis homeobox 2, Meis1 related protein 1, MEIS2, MRG1
Calculated Molecular Weight 381 aa, 42 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC001516
Gene Symbol MEIS2
Gene ID (NCBI) 4212
RRIDAB_2143028
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14770
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEIS2, also named as Meis1-related protein 1 or MRG1, is a 477 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the TALE/MEIS homeobox family. MEIS2 is expressed in various tissues and at high level in the lymphoid organs of hematopoietic tissues. MEIS2 localizes in the nucleus and is involved in transcriptional regulation. MEIS2 is a critical downstream effector within an essential homeoprotein network that serving a rate-limiting regulatory role in MLL leukemogenesis. MEIS2 as midbrain specific marker is upregulated concomitantly with the morphological transformation of hindbrain to midbrain.

实验方案

Product Specific Protocols
WB protocol for MEIS2 antibody 11550-1-APDownload protocol
IHC protocol for MEIS2 antibody 11550-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Proc Natl Acad Sci U S A

Congenital diaphragmatic hernia candidate genes derived from embryonic transcriptomes.

Authors - Russell Meaghan K MK
View Article