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验证数据展示

  • WB analysis of HepG2 using 28341-1-AP

    WB analysis of HepG2 using 28341-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 28341-1-AP (MFN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 28341-1-AP

    WB analysis using 28341-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28341-1-AP (MFN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 28341-1-AP

    IHC staining of mouse heart using 28341-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28341-1-AP (MFN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MFN2 antibody included
  • IHC staining of mouse heart using 28341-1-AP

    IHC staining of mouse heart using 28341-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28341-1-AP (MFN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MFN2 antibody included

MFN2 Polyclonal antibody

MFN2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28341-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Rat

应用

WB, IHC, ELISA

别名

CMT2A, CMT2A2, CPRP1, HSG, KIAA0214, MARF, MFN2, mitofusin 2, Transmembrane GTPase MFN2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MFN2 Ready-To-Use IHC Kit. MFN2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28341-1-AP targets MFN2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 MFN2 fusion protein Ag28128 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitofusin 2
别名 CMT2A, CMT2A2, CPRP1, HSG, KIAA0214, MARF, MFN2, mitofusin 2, Transmembrane GTPase MFN2
计算分子量 757 aa, 86 kDa
观测分子量 86 kDa
GenBank蛋白编号BC017061
基因名称 MFN2
Gene ID (NCBI) 9927
ENSEMBL Gene IDENSG00000116688
RRIDAB_2881116
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95140
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MFN2, also named as CPRP1 and KIAA0214, belongs to the mitofusin family. It is an Essential transmembrane GTPase, which mediates mitochondrial fusion. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression of MFN2 induces the formation of mitochondrial networks. It plays an important role in the regulation of vascular smooth muscle cell proliferation. Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 2A2 (CMT2A2). Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 6 (CMT6). Ubiquitinated forms of Mfn2 (mono- and polyubiquitinated) are present during mitophagy.

实验方案

Product Specific Protocols
WB protocol for MFN2 antibody 28341-1-APDownload protocol
IHC protocol for MFN2 antibody 28341-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols