验证数据展示
经过测试的应用
Positive WB detected in | MCF7 cells, mouse placenta tissue, MCF-7 cells, A549 cells, Jurkat cells, mouse brain tissue, rat brain tissue |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20292-1-AP targets MLN64 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | MLN64 fusion protein Ag14061 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | StAR-related lipid transfer (START) domain containing 3 |
Synonyms | CAB1, MLN 64, MLN64, Protein CAB1, STARD3 |
Calculated Molecular Weight | 445 aa, 51 kDa |
Observed Molecular Weight | 33-53 kDa |
GenBank Accession Number | BC008747 |
Gene Symbol | MLN64/STARD3 |
Gene ID (NCBI) | 10948 |
RRID | AB_10643978 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q14849 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MLN64 (also known as STARD3) is an integral membrane protein anchored in late endosomes. MLN64 has a StAR-related lipid-transfer (START) domain to bind cholesterol, mediating intracellular cholesterol transfer. MLN64 is widely expressed in multiple tissues including liver, spleen, heart, kidney, lung, and the brain. Higher expression of MLN64 has been found in several malignancies. MLN64 can be detected as 33 kDa truncated fragment (PMID:9237999, PMID:15718238).
实验方案
Product Specific Protocols | |
---|---|
WB protocol for MLN64 antibody 20292-1-AP | Download protocol |
IHC protocol for MLN64 antibody 20292-1-AP | Download protocol |
IF protocol for MLN64 antibody 20292-1-AP | Download protocol |
IP protocol for MLN64 antibody 20292-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Life Sci Blockade of STARD3-mediated cholesterol transport alleviates diabetes-induced podocyte injury by reducing mitochondrial cholesterol accumulation
| ||
J Cell Sci The lysosomal lipid transporter LIMP-2 is part of lysosome-ER STARD3-VAPB-dependent contact sites |