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MRI1 Polyclonal antibody

MRI1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19916-1-AP

Print datasheet

Synonyms

M1Pi, MRDI, MRI1, MTNA, MTR 1 P isomerase, Ypr118w

验证数据展示

  • WB analysis of HT-1080 using 19916-1-AP

    WB analysis of HT-1080 using 19916-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 19916-1-AP (MRI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of rat kidney using 19916-1-AP

    IHC staining of rat kidney using 19916-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19916-1-AP (MRI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat kidney using 19916-1-AP

    IHC staining of rat kidney using 19916-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19916-1-AP (MRI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHT-1080 cells, HL-60 cells
Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19916-1-AP targets MRI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MRI1 fusion protein Ag13809 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name methylthioribose-1-phosphate isomerase homolog (S. cerevisiae)
Synonyms M1Pi, MRDI, MRI1, MTNA, MTR 1 P isomerase, Ypr118w
Calculated Molecular Weight 369 aa, 39 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC001703
Gene Symbol MRI1
Gene ID (NCBI) 84245
RRIDAB_10638924
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BV20
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRI1 antibody 19916-1-APDownload protocol
IHC protocol for MRI1 antibody 19916-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols