验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, mouse thymus tissue, HEK-293 cells |
| Positive IP detected in | mouse thymus tissue |
| Positive IHC detected in | rat lung tissue, human heart tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy MTA2 Ready-To-Use IHC Kit. MTA2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17554-1-AP targets MTA2 in WB, IHC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, CoIP, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | MTA2 fusion protein Ag11713 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | metastasis associated 1 family, member 2 |
| 别名 | MTA1-L1 protein, MTA1L1, MTA1 L1 protein, Metastasis-associated protein MTA2, Metastasis-associated 1-like 1 |
| 计算分子量 | 668 aa, 75 kDa |
| 观测分子量 | 70-75 kDa |
| GenBank蛋白编号 | BC053650 |
| 基因名称 | MTA2 |
| Gene ID (NCBI) | 9219 |
| RRID | AB_10638601 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O94776 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Metastasis-associated protein MTA2, also named as MTA1L1 or PID, is a 668 amino acid protein, which contains one BAH domain, one ELM2 domain, one GATA-type zinc finger and one SANT domain. MTA2 localizes in the nucleus and is widely expressed in many tissues. MTA2 may be involved in the regulation of gene expression as repressor and activator.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MTA2 antibody 17554-1-AP | Download protocol |
| IHC protocol for MTA2 antibody 17554-1-AP | Download protocol |
| IP protocol for MTA2 antibody 17554-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Rep miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded multiplex RNAi at a single-cell level. | ||
Cell Mol Gastroenterol Hepatol Targeting HNRNPM Inhibits Cancer Stemness and Enhances Antitumor Immunity in Wnt-activated Hepatocellular Carcinoma.
| ||
Cancer Sci MTA2 promotes the metastasis of esophageal squamous cell carcinoma via EIF4E-Twist feedback loop. | ||
Nucleic Acids Res CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development |
