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MYBBP1A Polyclonal antibody

MYBBP1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14524-1-AP

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Synonyms

PAP2, Myb-binding protein 1A, Myb binding protein 1A



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MYBBP1A Ready-To-Use IHC Kit. MYBBP1A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14524-1-AP targets MYBBP1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen MYBBP1A fusion protein Ag6008 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MYB binding protein (P160) 1a
Synonyms PAP2, Myb-binding protein 1A, Myb binding protein 1A
Calculated Molecular Weight 149 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC050546
Gene Symbol MYBBP1A
Gene ID (NCBI) 10514
RRIDAB_2148137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQG0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The protooncogene MYB is predominantly expressed in immature hemopoietic cells where it has an essential role in hemopoietic cell proliferation and differentiation. Oncogenically activated forms of MYB is generally N- and/or C-terminal truncations of the normal MYB protein. Removal of the C terminus of MYB disrupts or deletes a region termed the negative regulatory domain (NRD), resulting in an increase in DNA binding, transactivation, and transformation by MYB. One feature of the NRD is a leucine zipper-like motif [PMID: 8302594]. Murine Myb-binding protein-1a (MYBBP1A), originally called P160, was identified by its ability to interact specifically with Myb via this leucine zipper-like motif. MYBBP1A modulates MYB activity upon binding to the MYB NRD [PMID: 10644447, 9447996].

实验方案

Product Specific Protocols
WB protocol for MYBBP1A antibody 14524-1-APDownload protocol
IHC protocol for MYBBP1A antibody 14524-1-APDownload protocol
IF protocol for MYBBP1A antibody 14524-1-APDownload protocol
IP protocol for MYBBP1A antibody 14524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Rep

A fetal tumor suppressor axis abrogates MLL-fusion-driven acute myeloid leukemia

Authors - Mohamed Eldeeb
humanWB

Life Sci Alliance

The nucleolar phase of signal recognition particle assembly

Authors - Amani Issa
humanIHC

Clin Genet

Non-immune hydrops fetalis is associated with bi-allelic pathogenic variants in the MYB Binding Protein 1a (MYBBP1A) gene

Authors - Jair Tenorio-Castano
mouseWB

Sci Adv

In vivo protein turnover rates in varying oxygen tensions nominate MYBBP1A as a mediator of the hyperoxia response

Authors - Xuewen Chen
humanWB

Microbiol Spectr

Nuclear reorganization by NPM1-mediated phase separation triggered by adenovirus core protein VII

Authors - Michelle Jane Genoveso
humanWB,IF

Cancers (Basel)

Loss of MYBBP1A Induces Cancer Stem Cell Activity in Renal Cancer.

Authors - Blanca Felipe-Abrio
  • KD Validated