NANOG Monoclonal antibody

NANOG Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A2E1

Cat no : 67255-1-Ig

Print datasheet

Synonyms

hNanog, Homeobox protein NANOG, NANOG, Nanog homeobox



经过测试的应用

Positive WB detected inHT-29 cells, HuH-7 cells, MDA-MB-231 cells, MCF-7 cells, T-47D cells, NCCIT cells, JAR cells, human placenta tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 13 publications below

产品信息

67255-1-Ig targets NANOG in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse
Cited Reactivityhuman
Immunogen NANOG fusion protein Ag21364 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name Nanog homeobox
Synonyms hNanog, Homeobox protein NANOG, NANOG, Nanog homeobox
Calculated Molecular Weight 35 kDa
Observed Molecular Weight35-40 kDa
GenBank Accession NumberBC160187
Gene Symbol NANOG
Gene ID (NCBI) 79923
RRIDAB_2882529
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H9S0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants could recognized by NANOG, one is normal form (~39kd), the other is post-translation modified form (~48kd) (21136380 ). Nanog exists two isoforms with molecular weight 34.4 kDa and 31.9 kDa. (PMID: 21969378)

实验方案

Product Specific Protocols
WB protocol for NANOG antibody 67255-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Pineal Res

Melatonin inhibits the stemness of head and neck squamous cell carcinoma by modulating HA synthesis via the FOSL1/HAS3 axis

Authors - Xinyue Luo
humanWB

iScience

Arl4c promotes the growth and drug resistance of pancreatic cancer by regulating tumor-stromal interactions.

Authors - Xin Chen
humanWB

Biomed Pharmacother

Limonin enhances the radiosensitivity of nasopharyngeal carcinoma cells via attenuating Stat3-induced cell stemness.

Authors - Ling Gao
humanWB

Med Sci Monit

PMID: 31760402

Authors - Xueni Liu
humanWB

Mol Carcinog

The small nucleolar RNA SNORA51 enhances breast cancer stem cell-like properties via the RPL3/NPM1/c-MYC pathway

Authors - Shan Li
humanWB

Cancer Lett

Loss of Claudin-1 incurred by DNMT aberration promotes pancreatic cancer progression

Authors - Linxi Zhu