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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 66924-1-Ig

    WB analysis using 66924-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66924-1-Ig (NFATC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

NFATC3 Monoclonal antibody

NFATC3 Monoclonal Antibody for WB, ELISA
Cat No. 66924-1-Ig

产品说明书

CloneNo. 1C1H8

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

NF AT4, NF ATc3, NFAT4, NFATC3, NFATX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, Daudi cells
Planning an IHC experiment? We recommend our IHCeasy NFATC3 Ready-To-Use IHC Kit. NFATC3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66924-1-Ig targets NFATC3 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 NFATC3 fusion protein Ag27306 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
亲和力
KD=1.16 x 10-10M
KOff=5.67 x 10-6M
KOn=4.90 x 104M
全称 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
别名 NF AT4, NF ATc3, NFAT4, NFATC3, NFATX
计算分子量 116 kDa
观测分子量 160 kDa
GenBank蛋白编号BC001050
基因名称 NFATC3
Gene ID (NCBI) 4775
RRIDAB_2882251
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ12968
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)

实验方案

Product Specific Protocols
WB protocol for NFATC3 antibody 66924-1-IgDownload protocol
IHC protocol for NFATC3 antibody 66924-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols