验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, mouse brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Planning an IHC experiment? We recommend our IHCeasy NFIC Ready-To-Use IHC Kit. NFIC primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16399-1-AP targets NFIC in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, bovine |
| 免疫原 | NFIC fusion protein Ag9494 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear factor I/C (CCAAT-binding transcription factor) |
| 别名 | NFI-C, NF-I/C, NF1-C, NF I, CTF |
| 计算分子量 | 428aa,48 kDa; 508aa,57 kDa |
| 观测分子量 | 56 kDa |
| GenBank蛋白编号 | BC012120 |
| 基因名称 | NFIC |
| Gene ID (NCBI) | 4782 |
| RRID | AB_2878252 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P08651 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NFIC, also named as Nuclear factor 1 C-type, is a 508 amino acid protein, which contains 1 CTF/NF-I DNA-binding domain and belongs to the CTF/NF-I family. NFIC localizes in the nucleus and recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NFIC antibody 16399-1-AP | Download protocol |
| IHC protocol for NFIC antibody 16399-1-AP | Download protocol |
| IF protocol for NFIC antibody 16399-1-AP | Download protocol |
| IP protocol for NFIC antibody 16399-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Bone Miner Res METTL3-mediated m6 A mRNA methylation modulates tooth root formation via affecting NFIC translation. | ||
J Agric Food Chem Knockdown of NFIC Promotes Bovine Myoblast Proliferation through the CENPF/CDK1 Axis
| ||
Cancer Cell Int NFIC mediates m6A mRNA methylation to orchestrate transcriptional and post-transcriptional regulation to represses malignant phenotype of non-small cell lung cancer cells | ||
Mol Carcinog Neuroplastin-β mediates S100A8/A9-induced lung cancer disseminative progression. |
