验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HT-1080 cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human colon cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
12981-1-AP targets NFYA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | NFYA fusion protein Ag3604 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear transcription factor Y, alpha |
| 别名 | NF-YA, NF YA, HAP2, CBF B, CBF A |
| 计算分子量 | 318aa,34 kDa; 347aa,39 kDa |
| 观测分子量 | 40 kDa, 46 kDa |
| GenBank蛋白编号 | BC039244 |
| 基因名称 | NFYA |
| Gene ID (NCBI) | 4800 |
| RRID | AB_10644128 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P23511 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NFYA, also named as Nuclear transcription factor Y subunit alpha, is a 347 amino acid protein, which contains 1 NFYA/HAP2-type DNA-binding domain and belongs to the NFYA/HAP2 subunit family. NFYA exists the two 41- to 45-kDa isoforms of NF-YA ( 11287613 ). NFYA is a component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NFYA antibody 12981-1-AP | Download protocol |
| IHC protocol for NFYA antibody 12981-1-AP | Download protocol |
| IF protocol for NFYA antibody 12981-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Virol Minute Virus of Mice (MVM) inhibits transcription of the cyclin B1 gene during infection. | ||
Int J Oncol Gene regulatory network construction identified NFYA as a diffuse subtype-specific prognostic factor in gastric cancer.
| ||
Cell Death Dis PRMT5 critically mediates TMAO-induced inflammatory response in vascular smooth muscle cells.
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