验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, mouse heart tissue, mouse placenta tissue |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13766-1-AP targets Entactin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | Entactin fusion protein Ag4749 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nidogen 1 |
| 别名 | NID1, NID, NID 1, NID-1, nidogen 1 |
| 计算分子量 | 1247 aa, 136 kDa |
| 观测分子量 | 136-150 kDa |
| GenBank蛋白编号 | BC045606 |
| 基因名称 | Entactin/NID |
| Gene ID (NCBI) | 4811 |
| RRID | AB_10642564 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P14543 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nidogen 1 (Entactin), first identified from a basement membrane-secreting cell line and the murine EHS tumor, is together with perlecan, laminin, and collagen IV, a ubiquitous component of basement membranes (PMID: 19530222). Nidogen-1 is made by mesenchymal cells and is incorporated into the epithelial BM. It is a sulfated glycoprotein of 150 kDa with three globular domains, G1, G2 and G3, and contains binding sites for other ECM molecules (PMID: 10958695, 10671374).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Entactin antibody 13766-1-AP | Download protocol |
| IHC protocol for Entactin antibody 13766-1-AP | Download protocol |
| IF protocol for Entactin antibody 13766-1-AP | Download protocol |
| IP protocol for Entactin antibody 13766-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Bioact Mater Nidogen1-enriched extracellular vesicles accelerate angiogenesis and bone regeneration by targeting Myosin-10 to regulate endothelial cell adhesion. | ||
Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility. | ||
Biomaterials Stiffness assisted cell-matrix remodeling trigger 3D mechanotransduction regulatory programs | ||
Signal Transduct Target Ther Human adipose and umbilical cord mesenchymal stem cell-derived extracellular vesicles mitigate photoaging via TIMP1/Notch1 |
