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NPTX1 Polyclonal antibody
NPTX1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IF-P, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, HepG2 cells, mouse liver tissue, mouse cerebellum tissue, rat cerebellum tissue |
Positive IHC detected in | mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | rat brain tissue |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20656-1-AP targets NPTX1 in WB, IHC, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
Cited Applications | WB, IF, CoIP, ELISA |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | neuronal pentraxin I |
Synonyms | NP-I, NP1, Neuronal pentraxin-1, Neuronal pentraxin I |
Calculated Molecular Weight | 47 kDa |
Observed Molecular Weight | 47 kDa |
GenBank Accession Number | NM_002522 |
Gene Symbol | NPTX1 |
Gene ID (NCBI) | 4884 |
RRID | AB_10793669 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q15818 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for NPTX1 antibody 20656-1-AP | Download protocol |
IHC protocol for NPTX1 antibody 20656-1-AP | Download protocol |
IF protocol for NPTX1 antibody 20656-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Immunol Identification of Neuronal Pentraxins as Synaptic Binding Partners of C1q and the Involvement of NP1 in Synaptic Pruning in Adult Mice. | ||
Acta Biochim Biophys Sin (Shanghai) miR-139-5p mediates the palmitate-induced inhibition of insulin secretion by targeting neuronal pentraxin 1 in INS-1 cells. | ||
PLoS One RNA-seq analysis of gene expression profiles in isolated stria vascularis from wild-type and Alport mice reveals key pathways underling Alport strial pathogenesis. | ||
Cancer Manag Res NPTX1 promotes metastasis via integrin/FAK signaling in gastric cancer.
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PLoS One Exosome-delivered circRPS5 inhibits the progression of melanoma via regulating the miR-151a/NPTX1 axis
| ||
Oncogene Chromatin-associated OGT promotes the malignant progression of hepatocellular carcinoma by activating ZNF263 |