验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HepG2 cells, mouse liver tissue, mouse cerebellum tissue, rat cerebellum tissue |
| Positive IHC detected in | mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20656-1-AP targets NPTX1 in WB, IHC, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| Cited Applications | WB, IF, CoIP, ELISA |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | neuronal pentraxin I |
| Synonyms | NP-I, NP1, Neuronal pentraxin-1, Neuronal pentraxin I |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | NM_002522 |
| Gene Symbol | NPTX1 |
| Gene ID (NCBI) | 4884 |
| RRID | AB_10793669 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15818 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NPTX1, also named as NP1, may mediate uptake of degraded synaptic material which could play an important role in synaptic remodeling. NPTX1 can mediate the neuronal and glial uptake of the snake venom toxin taipoxin. The antibody is specific to NPTX1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NPTX1 antibody 20656-1-AP | Download protocol |
| IHC protocol for NPTX1 antibody 20656-1-AP | Download protocol |
| IF protocol for NPTX1 antibody 20656-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Immunol Identification of Neuronal Pentraxins as Synaptic Binding Partners of C1q and the Involvement of NP1 in Synaptic Pruning in Adult Mice. | ||
Acta Biochim Biophys Sin (Shanghai) miR-139-5p mediates the palmitate-induced inhibition of insulin secretion by targeting neuronal pentraxin 1 in INS-1 cells. | ||
PLoS One RNA-seq analysis of gene expression profiles in isolated stria vascularis from wild-type and Alport mice reveals key pathways underling Alport strial pathogenesis. | ||
Cancer Manag Res NPTX1 promotes metastasis via integrin/FAK signaling in gastric cancer.
| ||
PLoS One Exosome-delivered circRPS5 inhibits the progression of melanoma via regulating the miR-151a/NPTX1 axis
| ||
Oncogene Chromatin-associated OGT promotes the malignant progression of hepatocellular carcinoma by activating ZNF263 |
