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验证数据展示

  • WB analysis of human liver using 19709-1-AP

    WB analysis of human liver using 19709-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 19709-1-AP (neurturin-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 19709-1-AP

    IHC staining of mouse brain using 19709-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19709-1-AP (neurturin-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 19709-1-AP

    IHC staining of mouse brain using 19709-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19709-1-AP (neurturin-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Neurturin Polyclonal antibody

Neurturin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19709-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

neurturin-Specific, NRTN

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman liver tissue, human heart tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19709-1-AP targets Neurturin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neurturin
Synonyms neurturin-Specific, NRTN
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberNM_004558
Gene Symbol Neurturin
Gene ID (NCBI) 4902
RRIDAB_10644284
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99748
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NRTN belongs to the TGF-beta family and GDNF subfamily. NRTN supports the survival of sympathetic neurons in culture. NRTN may regulate the development and maintenance of the CNS. It might control the size of non-neuronal cell population such as haemopoietic cells. Defects in NRTN are a cause of Hirschsprung disease (HSCR). The antibody is specific to NRTN.

实验方案

Product Specific Protocols
WB protocol for Neurturin antibody 19709-1-APDownload protocol
IHC protocol for Neurturin antibody 19709-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols