NUP98-NUP96 Polyclonal antibody

NUP98-NUP96 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12329-1-AP

Print datasheet

Synonyms

NUP98 NUP96, Nuclear pore complex protein Nup98-Nup96, Nuclear pore complex protein Nup98, Nuclear pore complex protein Nup96, EC:3.4.21.-



经过测试的应用

Positive WB detected inCOLO 320 cells, Jurkat cells, HeLa cells, K-562 cells, MCF-7 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12329-1-AP targets NUP98-NUP96 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen NUP98-NUP96 fusion protein Ag3016 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nucleoporin 98kDa
Synonyms NUP98 NUP96, Nuclear pore complex protein Nup98-Nup96, Nuclear pore complex protein Nup98, Nuclear pore complex protein Nup96, EC:3.4.21.-
Calculated Molecular Weight 98 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC012906
Gene Symbol NUP98
Gene ID (NCBI) 4928
RRIDAB_10973678
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52948
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nup98-Nup96 (also known as Nup196, ADIR2 or ADAR2), encoded by NUP98 gene, is a component of the nuclear pore complex (NPC) . It is first synthesized as 186-198 kDa Nup98-Nup96 precursors and the precursors shortly undergo autoproteolysis to give rise to two nucleoporins, the amino-terminal Nup98 (Nup98-N) and carboxy-terminal Nup96 (Nup96-C). Both of Nup98 and Nup96 are localized to the nucleoplasmic side of the NPC. This antibody was raised against the carboxy-terminal region of Nup98-Nup96, it recognizes all Nup98-Nup96 proteins except Nup98-N. Since the autoproteolysis is very rapid this antibody usually detected 98-115 kDa Nup96 protein in multiple cell lines. It is notable that RNA-editing deaminase 1 (Gene ID: 104), also named ADAR2, is a different protein from Nup98-Nup96 (ADAR2).

实验方案

Product Specific Protocols
WB protocol for NUP98-NUP96 antibody 12329-1-APDownload protocol
IHC protocol for NUP98-NUP96 antibody 12329-1-APDownload protocol
IF protocol for NUP98-NUP96 antibody 12329-1-APDownload protocol
IP protocol for NUP98-NUP96 antibody 12329-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Neuron

Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination.

Authors - Zhixiong Liu
humanIF

Cell Rep

Antiviral Immune Response as a Trigger of FUS Proteinopathy in Amyotrophic Lateral Sclerosis.

Authors - Tatyana A Shelkovnikova
humanIF

Nat Commun

iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy

Authors - Vincent Louvel