验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14214-1-AP targets OAS2 in WB, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | human |
Immunogen | OAS2 fusion protein Ag5436 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | 2'-5'-oligoadenylate synthetase 2, 69/71kDa |
Synonyms | (2 5)oligo(A) synthase 2, 2 5 oligoadenylate synthase 2, 2 5A synthase 2, OAS2, p69 OAS / p71 OAS, p69OAS / p71OAS |
Calculated Molecular Weight | 82 kDa |
Observed Molecular Weight | 66-71 kDa |
GenBank Accession Number | BC049215 |
Gene Symbol | OAS2 |
Gene ID (NCBI) | 4939 |
RRID | AB_10892671 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P29728 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS2, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS2 is also named as p69 OAS/p71 OAS. It has 3 isoforms(71/69/20 kDa) produced by alternative splicing. The OAS2 isozymes are 69-71 kDa and form dimers(PMID:17765707). Glycosylation is essential for its activity(PMID:9880569). Constitutive expression of the 69-kDa form of the OAS2 protein in human cells inhibited the replication of encephalomyocarditis virus (EMCV) but not that of VSV, Sendai virus, or reovirus(PMID:16501108). This antibody recognizes isoform p71 and isoform p69 of OAS2.
实验方案
Product Specific Protocols | |
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WB protocol for OAS2 antibody 14214-1-AP | Download protocol |
IHC protocol for OAS2 antibody 14214-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |