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验证数据展示

  • WB analysis of Jurkat using 14214-1-AP

    WB analysis of Jurkat using 14214-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14214-1-AP (OAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

OAS2 Polyclonal antibody

OAS2 Polyclonal Antibody for WB, ELISA
Cat No. 14214-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

(2 5)oligo(A) synthase 2, 2 5 oligoadenylate synthase 2, 2 5A synthase 2, OAS2, p69 OAS / p71 OAS, p69OAS / p71OAS

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14214-1-AP targets OAS2 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen OAS2 fusion protein Ag5436 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 2'-5'-oligoadenylate synthetase 2, 69/71kDa
Synonyms (2 5)oligo(A) synthase 2, 2 5 oligoadenylate synthase 2, 2 5A synthase 2, OAS2, p69 OAS / p71 OAS, p69OAS / p71OAS
Calculated Molecular Weight 82 kDa
Observed Molecular Weight 66-71 kDa
GenBank Accession NumberBC049215
Gene Symbol OAS2
Gene ID (NCBI) 4939
RRIDAB_10892671
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29728
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS2, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS2 is also named as p69 OAS/p71 OAS. It has 3 isoforms(71/69/20 kDa) produced by alternative splicing. The OAS2 isozymes are 69-71 kDa and form dimers(PMID:17765707). Glycosylation is essential for its activity(PMID:9880569). Constitutive expression of the 69-kDa form of the OAS2 protein in human cells inhibited the replication of encephalomyocarditis virus (EMCV) but not that of VSV, Sendai virus, or reovirus(PMID:16501108). This antibody recognizes isoform p71 and isoform p69 of OAS2.

实验方案

Product Specific Protocols
WB protocol for OAS2 antibody 14214-1-APDownload protocol
IHC protocol for OAS2 antibody 14214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols