ORAI3 Polyclonal antibody

ORAI3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25766-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA and More (3)

Protein orai-3, TMEM142C, Transmembrane protein 142C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse heart tissue, BxPC-3 cells, LNCaP cells, PC-3 cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25766-1-AP targets ORAI3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 ORAI3 fusion protein Ag22513 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ORAI calcium release-activated calcium modulator 3
别名 Protein orai-3, TMEM142C, Transmembrane protein 142C
计算分子量 295 aa, 32 kDa
观测分子量 ~30 kDa, 60 kDa
GenBank蛋白编号BC006126
基因名称 ORAI3
Gene ID (NCBI) 93129
RRIDAB_2880231
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRQ5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ORAI3, also named TMEM142C, is a key regulator or component of store-operated Ca2+ channels and transcription factor NFAT nuclear import. ORAI3 channels appeared to differ from Orai1 and -2 in being somewhat resistant to Ca2+ depotentiation. The molecular weight of monomers and dimers for Orai3 is about 30-35 kDa and 60-70 kDa (PMID: 20971921; 17293345; 22580508).

实验方案

Product Specific Protocols
WB protocol for ORAI3 antibody 25766-1-APDownload protocol
IHC protocol for ORAI3 antibody 25766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Signal

ALOX5 drives the pyroptosis of CD4+ T cells and tissue inflammation in rheumatoid arthritis

Authors - Hao Cai
humanWB,IF,CoIP

BMJ Open Diabetes Res Care

Orai-IGFBP3 signaling complex regulates high-glucose exposure-induced increased proliferation, permeability, and migration of human coronary artery endothelial cells.

Authors - Suwen Bai
mouseIP

BMJ Open Diabetes Res Care

Orai-vascular endothelial-cadherin signaling complex regulates high-glucose exposure-induced increased permeability of mouse aortic endothelial cells.

Authors - Yuan Wei
humanWB

Exp Ther Med

Resveratrol sensitizes A549 cells to irradiation damage via suppression of store-operated calcium entry with Orai1 and STIM1 downregulation.

Authors - Wu Lele
ratWB

Allergy Asthma Immunol Res

Exercise Reduces Airway Smooth Muscle Contraction in Asthmatic Rats via Inhibition of IL-4 Secretion and Store-Operated Ca2+ Entry Pathway

Authors - Jun-Hao Huang
mouseIF

J Orthop Translat

Tibial cortex transverse transport regulates Orai1/STIM1-mediated NO release and improve the migration and proliferation of vessels via increasing osteopontin expression

Authors - Lingchao Kong
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