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  • KD/KO Validated

OXTR Polyclonal antibody

OXTR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 23045-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Oxytocin receptor, OT-R, OT R

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, L02 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman hysteromyoma tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23045-1-AP targets OXTR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human
Cited Reactivityhuman, mouse, rat, pig
Immunogen OXTR fusion protein Ag19074 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name OXTR
Synonyms Oxytocin receptor, OT-R, OT R
Calculated Molecular Weight 389 aa, 43 kDa
Observed Molecular Weight 46 kDa, 67 kDa
GenBank Accession NumberBC137443
Gene Symbol OXTR
Gene ID (NCBI) 5021
RRIDAB_2827425
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30559
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Oxytocin (OXT) is a neurohypophysial hormone that plays a role in lactation and parturition and in the central nervous system as a neurotransmitter involved in sex and maternal behavior (PMID: 1852313; 10811917). OXT acts through its receptor, OXTR, which belongs to the G protein-coupled 7-transmembrane receptor family. OXTR is expressed in the endometrium, myometrium, and mammary gland (PMID: 1313946). This antibody is raised against the C-terminal region of human OXTR. Two immunoreactive OXTR bands were found in the fat tissue, an unglycosylated 43 kDa form and a mature glycosylated 67 kDa form (PMID:32819381).

实验方案

Product Specific Protocols
WB protocol for OXTR antibody 23045-1-APDownload protocol
IHC protocol for OXTR antibody 23045-1-APDownload protocol
IF protocol for OXTR antibody 23045-1-APDownload protocol
IP protocol for OXTR antibody 23045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Nat Commun

PMID: 24915299

Authors - Christian Elabd
mouseIF

Acta Physiol (Oxf)

Oxytocin modulates neural processing of mitral/tufted cells in the olfactory bulb.

Authors - Changcheng Sun
mouseIF

Oxid Med Cell Longev

PMID: 32082478

Authors - Daniela C Wigger
mouseIHC

Antioxidants (Basel)

ΔMST and the Regulation of Cardiac CSE and OTR Expression in Trauma and Hemorrhage.

Authors - Britta Trautwein
humanWB

Biochem Pharmacol

Synergistic antitumor activity of 5-fluorouracil and atosiban against microsatellite stable colorectal cancer through restoring GATA3.

Authors - Mengmeng Wang
  • KD Validated
humanWB

Front Pharmacol

The Role of Formyl Peptide Receptor 1 in Uterine Contraction During Parturition.

Authors - Chaolu Chen
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