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验证数据展示

  • WB analysis of Jurkat using 18273-1-AP

    WB analysis of Jurkat using 18273-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18273-1-AP (P2RY1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 18273-1-AP

    IHC staining of human testis using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 18273-1-AP

    IHC staining of human testis using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 40x lens).

P2RY1 Polyclonal antibody

P2RY1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18273-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ATP receptor, P2RY1, P2Y purinoceptor 1, P2Y1, Purinergic receptor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated

规格: 
50 μL
100 μL
150 μL

¥2200/ 100 μL

浓度: 300 μg/ml

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经过测试的应用

Positive WB detected inJurkat cells, mouse heart tissue
Positive IHC detected inhuman testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18273-1-AP targets P2RY1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 P2RY1 fusion protein Ag12992 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 purinergic receptor P2Y, G-protein coupled, 1
别名 ATP receptor, P2RY1, P2Y purinoceptor 1, P2Y1, Purinergic receptor
计算分子量 373 aa, 42 kDa
观测分子量 42 kDa, 57 kDa-59 kDa, 66 kDa
GenBank蛋白编号BC074785
基因名称 P2RY1
Gene ID (NCBI) 5028
RRIDAB_2158402
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP47900
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for P2RY1 antibody 18273-1-APDownload protocol
IHC protocol for P2RY1 antibody 18273-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols