PCP4 Polyclonal antibody

PCP4 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14705-1-AP

Print datasheet

Synonyms

Purkinje cell protein 4, PEP19, PEP 19, Calmodulin regulator protein PCP4, Brain-specific polypeptide PEP-19



经过测试的应用

Positive WB detected inmouse brain tissue, rat brain
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman cerebellum tissue, mouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy PCP4 Ready-To-Use IHC Kit. PCP4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14705-1-AP targets PCP4 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen PCP4 fusion protein Ag6388 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Purkinje cell protein 4
Synonyms Purkinje cell protein 4, PEP19, PEP 19, Calmodulin regulator protein PCP4, Brain-specific polypeptide PEP-19
Calculated Molecular Weight 7 kDa
Observed Molecular Weight 8 kDa
GenBank Accession NumberBC013791
Gene Symbol PCP4
Gene ID (NCBI) 5121
RRIDAB_2878075
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48539
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCP4, also named as PEP19, belongs to a family of proteins involved in calcium transduction signals and binds calmodulin via an IQ motif, in a calcium independent manner. It is mainly expressed in ectoderm and neuroectoderm comprising neural crest derived cells.

实验方案

Product Specific Protocols
WB protocol for PCP4 antibody 14705-1-APDownload protocol
IHC protocol for PCP4 antibody 14705-1-APDownload protocol
IF protocol for PCP4 antibody 14705-1-APDownload protocol
IP protocol for PCP4 antibody 14705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cancer Cell

Single-Cell Characterization of Malignant Phenotypes and Developmental Trajectories of Adrenal Neuroblastoma.

Authors - Rui Dong
humanIF

Cell Stem Cell

Human brain organoids assemble functionally integrated bilateral optic vesicles.

Authors - Elke Gabriel
humanWB

Ann Neurol

PMID: 32167609

Authors - Jimena Monzón-Sandoval
ratIF

Nat Neurosci

Acute silencing of hippocampal CA3 reveals a dominant role in place field responses.

Authors - Heydar Davoudi
humanIHC

Biomedicines

Characteristic Analysis of Featured Genes Associated with Cholangiocarcinoma Progression

Authors - Qigu Yao
humanIF

Nat Protoc

Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia

Authors - Elke Gabriel