验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, Y79 cells, K-562 cells, L02 cells |
| Positive IP detected in | HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20359-1-AP targets PER2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IP, ELISA Application Description |
| Cited Applications | WB, IP, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, goat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | period homolog 2 (Drosophila) |
| Synonyms | FASPS, hPER2, KIAA0347, PER2, period homolog 2 (Drosophila) |
| Calculated Molecular Weight | 137 kDa |
| Observed Molecular Weight | 136 kDa |
| GenBank Accession Number | NM_022817 |
| Gene Symbol | PER2 |
| Gene ID (NCBI) | 8864 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15055 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. PER2 is a negative element in the circadian transcriptional loop. PER2 influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. It negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. The antibody is specific to PER2. It can recognize isoform1 and isoform2 of PER2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PER2 antibody 20359-1-AP | Download protocol |
| IP protocol for PER2 antibody 20359-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Time-of-day specificity of anticancer drugs may be mediated by circadian regulation of the cell cycle. | ||
Nat Commun Correspondence: Oncogenic MYC persistently upregulates the molecular clock component REV-ERBα. | ||
Elife NRF2 regulates core and stabilizing circadian clock loops, coupling redox and timekeeping inMus musculus. |
