验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, K-562 cells, mouse heart tissue |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14713-1-AP targets PEX19 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, arabidopsis |
| 免疫原 | PEX19 fusion protein Ag6434 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | peroxisomal biogenesis factor 19 |
| 别名 | 33 kDa housekeeping protein, D1S2223E, HK33, Peroxin 19, PEX19, PMP1, PMPI, PXF, PXMP1 |
| 计算分子量 | 33 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC000496 |
| 基因名称 | PEX19 |
| Gene ID (NCBI) | 5824 |
| RRID | AB_2162265 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P40855 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. PEX19 gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs) (PMID: 14709540). PEX19 may bind newly synthesized PMPs and facilitate their insertion into the peroxisome membrane (PMID: 107044440. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. Defects in this gene are a cause of Zellweger syndrome (ZWS), as well as peroxisome biogenesis disorder complementation group 14 (PBD-CG14) (PMID:20683989) (PMID:10051604).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PEX19 antibody 14713-1-AP | Download protocol |
| IHC protocol for PEX19 antibody 14713-1-AP | Download protocol |
| IP protocol for PEX19 antibody 14713-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Limited survival and impaired hepatic fasting metabolism in mice with constitutive Rag GTPase signaling. | ||
Cell Death Differ Peroxisome-driven ether-linked phospholipids biosynthesis is essential for ferroptosis. | ||
Cell Commun Signal TFEB activation triggers pexophagy for functional adaptation during oxidative stress under calcium deficient-conditions | ||
Cell Mol Life Sci Dietary cysteine and methionine promote peroxisome elevation and fat loss by induction of CG33474 expression in Drosophila adipose tissue |
