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Pepsinogen II Polyclonal antibody

Pepsinogen II Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16881-1-AP

Print datasheet

Synonyms

PGC, PGⅡ, EC:3.4.23.3

验证数据展示

  • WB analysis of human stomach using 16881-1-AP

    WB analysis of human stomach using 16881-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 16881-1-AP (Pepsinogen II antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 16881-1-AP

    IHC staining of human stomach cancer using 16881-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16881-1-AP (Pepsinogen II antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman stomach tissue, mouse stomach tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16881-1-AP targets Pepsinogen II in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Pepsinogen II fusion protein Ag10332 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name progastricsin (pepsinogen C)
Synonyms PGC, PGⅡ, EC:3.4.23.3
Calculated Molecular Weight 388 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC073740
Gene Symbol PGC
Gene ID (NCBI) 5225
RRIDAB_10596919
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20142
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pepsinogen II also known as progastricsin or PGC(pepsinogen C), is an aspartic protease expressed primarily in gastric chief cells and is a novel marker of type 2 cells with advantages over many of the current markers used to identify type 2 cells in the developing lung(PMID:14578117). It is involved in proteolysis and peptidolysis. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for Pepsinogen II antibody 16881-1-APDownload protocol
IHC protocol for Pepsinogen II antibody 16881-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols