验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HEK-293 cells, mouse brain tissue, HeLa cells, HepG2 cells, MCF-7 cells, MDA-MB-453s cells, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lymphoma tissue, human breast hyperplasia tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14719-1-AP targets PHGDH in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PHGDH fusion protein Ag6445 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphoglycerate dehydrogenase |
| 别名 | EC:1.1.1.399, EC:1.1.1.37, D-3-phosphoglycerate dehydrogenase, 3-PGDH, 3PGDH |
| 计算分子量 | 57 kDa |
| 观测分子量 | 57 kDa |
| GenBank蛋白编号 | BC000303 |
| 基因名称 | PHGDH |
| Gene ID (NCBI) | 26227 |
| RRID | AB_2283938 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43175 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PHGDH(D-3-phosphoglycerate dehydrogenase) is also named as 3-PGDH, PGDH3 and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. 3-PGDH deficiency is a rare recessive inborn error in the biosynthesis of the amino acid L-serine characterized clinically by congenital microcephaly, psychomotor retardation, and intractable seizures(PMID:19235232 ).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PHGDH antibody 14719-1-AP | Download protocol |
| IHC protocol for PHGDH antibody 14719-1-AP | Download protocol |
| IF protocol for PHGDH antibody 14719-1-AP | Download protocol |
| IP protocol for PHGDH antibody 14719-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab PHGDH expression increases with progression of Alzheimer's disease pathology and symptoms. | ||
Nat Immunol PERK is a critical metabolic hub for immunosuppressive function in macrophages. | ||
Nat Commun Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence | ||
Mol Cell Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation | ||
Mol Cancer CircMYH9 drives colorectal cancer growth by regulating serine metabolism and redox homeostasis in a p53-dependent manner | ||
J Extracell Vesicles Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway. |
