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验证数据展示

  • WB analysis of HL-60 using 18469-1-AP

    WB analysis of HL-60 using 18469-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 18469-1-AP (PIAS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 18469-1-AP

    IF Staining of HeLa using 18469-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PIAS4 antibody (18469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 18469-1-AP

    FC experiment of HeLa using 18469-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PIAS4 (18469-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

PIAS4 Polyclonal antibody

PIAS4 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 18469-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

PIASy, PIAS-gamma, PIASG, PIAS gamma, EC:2.3.2.27

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18469-1-AP targets PIAS4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PIAS4 fusion protein Ag13316 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein inhibitor of activated STAT, 4
Synonyms PIASy, PIAS-gamma, PIASG, PIAS gamma, EC:2.3.2.27
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57-70 kDa
GenBank Accession NumberBC010047
Gene Symbol PIAS4
Gene ID (NCBI) 51588
RRIDAB_2164380
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N2W9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIAS4(protein inhibitor of activated STAT protein 4) is also named as PIASG, PIASy and belongs to the PIAS family. It enhanced the conjugation of SUMO2 to GATA2 through its E3 SUMO ligase activity. TGF-b can up-regulates PLAS4 expression in MCs to down-regulatea-SMA gene transcription by the interaction with E12 and PLAS4 predominantly acts as E3 ligases for E12 in MCs among PIAS family members(PMID:22829926). This protein can be sumoylated and Lys-35 is the main site of sumoylation(PMID:15831457).

实验方案

Product Specific Protocols
WB protocol for PIAS4 antibody 18469-1-APDownload protocol
IF protocol for PIAS4 antibody 18469-1-APDownload protocol
FC protocol for PIAS4 antibody 18469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols