验证数据展示

WB analysis of HEK-293 using 10495-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10495-1-AP
WB result of PIN1 antibody (10495-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PIN1 transfected HeLa cells.
IP experiment of HepG2 using 10495-1-AP
IP result of anti-PIN1 (IP:10495-1-AP, 4ug; Detection:10495-1-AP 1:500) with HepG2 cells lysate 2400ug.
IF Staining of HEK-293 using 10495-1-AP
Immunofluorescent analysis of HEK-293 cells using 10495-1-AP (PIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
WB analysis using 10495-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of human renal cell carcinoma using 10495-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10495-1-AP (PIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PIN1 antibody included
IHC staining of human renal cell carcinoma using 10495-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10495-1-AP (PIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PIN1 antibody included
IHC staining of human pancreas cancer using 10495-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10495-1-AP (PIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PIN1 antibody included
IHC staining of human pancreas cancer using 10495-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10495-1-AP (PIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PIN1 antibody included
IF Staining of HEK-293 using 10495-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PIN1 antibody (10495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of NIH/3T3 using 10495-1-AP
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PIN1 antibody (10495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HEK-293 using 10495-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PIN1 antibody (10495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO验证

PIN1 Polyclonal antibody

PIN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10495-1-AP

产品说明书

引用文献(38)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

Pin 1, Peptidyl-prolyl cis-trans isomerase Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC:5.2.1.8

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格:

-/ -

点击咨询售前客服

点击咨询售后客服

概述

经过测试的应用
产品信息
发表文章 (38)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A549 cells, HEK-293 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, PC-12 cells, mouse brain tissue, rat brain tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human renal cell carcinoma tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells, NIH/3T3 cells

Planning an IHC experiment? We recommend our IHCeasy PIN1 Ready-To-Use IHC Kit. PIN1 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 11 publications below
WB	See 34 publications below
IHC	See 9 publications below
IF	See 10 publications below
IP	See 2 publications below
CoIP	See 1 publications below

产品信息

10495-1-AP targets PIN1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat
免疫原	PIN1 fusion protein Ag0767 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	peptidylprolyl cis/trans isomerase, NIMA-interacting 1
别名	Pin 1, Peptidyl-prolyl cis-trans isomerase Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC:5.2.1.8
计算分子量	18 kDa
观测分子量	18 kDa
GenBank蛋白编号	BC002899
基因名称	PIN1
Gene ID (NCBI)	5300
RRID	AB_2163943
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q13526
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

• PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus. Pin1 inhibition simultaneously blocks multiple cancer pathways, disrupts the desmoplastic and immunosuppressive TME, and upregulates PD-L1 and ENT1, rendering pancreatic ductal adenocarcinoma (PDAC) eradicable by immunochemotherapy (PMID: 34388391).

实验方案

Product Specific Protocols
WB protocol for PIN1 antibody 10495-1-AP	Download protocol
IHC protocol for PIN1 antibody 10495-1-AP	Download protocol
IF protocol for PIN1 antibody 10495-1-AP	Download protocol
IP protocol for PIN1 antibody 10495-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB,IF,IP	Nat Commun Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells Authors - Qiuhong Zhu View Article
human,mouse	WB	J Pineal Res Melatonin directly binds and inhibits death-associated protein kinase 1 function in Alzheimer's disease. Authors - Dongmei Chen View Article
human	WB,IF	J Hazard Mater Cobalt induces neurodegenerative damages through Pin1 inactivation in mice and human neuroglioma cells. Authors - Fuli Zheng View Article
human	WB	Theranostics SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma. Authors - Ling Lin View Article
human	WB	Nucleic Acids Res Pin1 promotes GR transactivation by enhancing recruitment to target genes. Authors - Poolman Toryn M TM KD Validated View Article
mouse	WB, IHC, IF	Oxid Med Cell Longev PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway. Authors - Yanzhuo Zhang View Article

验证数据展示

WB analysis of HEK-293 using 10495-1-AP

WB analysis of HeLa using 10495-1-AP

IP experiment of HepG2 using 10495-1-AP

IF Staining of HEK-293 using 10495-1-AP

WB analysis using 10495-1-AP

IHC staining of human renal cell carcinoma using 10495-1-AP

IHC staining of human renal cell carcinoma using 10495-1-AP

IHC staining of human pancreas cancer using 10495-1-AP

IHC staining of human pancreas cancer using 10495-1-AP

IF Staining of HEK-293 using 10495-1-AP

IF Staining of NIH/3T3 using 10495-1-AP

IF Staining of HEK-293 using 10495-1-AP

PIN1 Polyclonal antibody

PIN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells

Melatonin directly binds and inhibits death-associated protein kinase 1 function in Alzheimer's disease.

Cobalt induces neurodegenerative damages through Pin1 inactivation in mice and human neuroglioma cells.

SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma.

Pin1 promotes GR transactivation by enhancing recruitment to target genes.

PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway.