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- KD/KO Validated
PKM1-specific Polyclonal antibody
PKM1-specific Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse skeletal muscle tissue, multi-cells/tissue, mouse muscle/liver tissues, mouse heart tissue, 293 cell, HepG2/MCF7 cells, mouse colon tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue, human skeletal muscle tissue, human brain tissue, human heart tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PKM2 Ready-To-Use IHC Kit. PKM2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15821-1-AP targets PKM1-specific in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey, bovine |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | PKM pyruvate kinase, muscle |
| Synonyms | CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM1, PK-M1, PKM1 pyruvate kinase, muscle, PKM1-specific, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK |
| Calculated Molecular Weight | 58 kDa |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | NM_182470 |
| Gene Symbol | PKM |
| Gene ID (NCBI) | 5315 |
| RRID | AB_2163820 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14618 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). This antibody is specific to PKM1 isoform.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PKM1-specific antibody 15821-1-AP | Download protocol |
| IHC protocol for PKM1-specific antibody 15821-1-AP | Download protocol |
| IP protocol for PKM1-specific antibody 15821-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.
| ||
Nat Immunol NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death. | ||
Nat Commun Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence | ||
Nat Commun Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2. | ||
Nat Commun The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis. | ||
Nat Commun Pyruvate kinase type M2 promotes tumour cell exosome release via phosphorylating synaptosome-associated protein 23. |
