验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PLD1 Ready-To-Use IHC Kit. PLD1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
18355-1-AP targets PLD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | PLD1 fusion protein Ag13227 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | phospholipase D1, phosphatidylcholine-specific |
| Synonyms | PLD 1, Phospholipase D1, Phosphatidylcholine-hydrolyzing phospholipase D1, hPLD1, EC:3.1.4.4 |
| Calculated Molecular Weight | 1074 aa, 124 kDa |
| Observed Molecular Weight | 112-124 kDa |
| GenBank Accession Number | BC068976 |
| Gene Symbol | PLD1 |
| Gene ID (NCBI) | 5337 |
| RRID | AB_2163859 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13393 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLD1(Phospholipase D1) belongs to the phospholipase D family. It is implicated in the release process of a variety of hormones, most likely at a late stage of exocytosis through the production of lipids that are essential for the fusion process and seem to be also essential for fast neurotransmitter release. PLD1 is primarily localized in vesicular structures such as endosomes, lysosomes, and autophagosomes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLD1 antibody 18355-1-AP | Download protocol |
| IHC protocol for PLD1 antibody 18355-1-AP | Download protocol |
| IF protocol for PLD1 antibody 18355-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |