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PLEKHM1 Polyclonal antibody

PLEKHM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16202-1-AP

Print datasheet

Synonyms

162 kDa adapter protein, AP162, B2, KIAA0356, OPTB6, PLEKHM1

验证数据展示

  • WB analysis of ROS1728 using 16202-1-AP

    WB analysis of ROS1728 using 16202-1-AP

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 16202-1-AP (PLEKHM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16202-1-AP

    IHC staining of human kidney using 16202-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 16202-1-AP

    IHC staining of human kidney using 16202-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inROS1728 cells, mouse liver tissue
Positive IHC detected inhuman kidney tissue, human liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

16202-1-AP targets PLEKHM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen PLEKHM1 fusion protein Ag9145 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pleckstrin homology domain containing, family M (with RUN domain) member 1
Synonyms 162 kDa adapter protein, AP162, B2, KIAA0356, OPTB6, PLEKHM1
Calculated Molecular Weight 117 kDa
Observed Molecular Weight 117 kDa
GenBank Accession NumberBC064361
Gene Symbol PLEKHM1
Gene ID (NCBI) 9842
RRIDAB_2878228
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4G2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLEKHM1 antibody 16202-1-APDownload protocol
IHC protocol for PLEKHM1 antibody 16202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Autophagy

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng
View Article
mouseWB

Nutrients

Improving Lipophagy by Restoring Rab7 Cycle: Protective Effects of Quercetin on Ethanol-Induced Liver Steatosis.

Authors - Hongkun Lin
View Article