SKAR Polyclonal antibody
SKAR Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, HepG2 cells, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy POLDIP3/SKAR Ready-To-Use IHC Kit. POLDIP3/SKAR primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
17466-1-AP targets SKAR in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | SKAR fusion protein Ag11338 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | polymerase (DNA-directed), delta interacting protein 3 |
| Synonyms | KIAA1649, p46, PDIP46, POLDIP3, S6K1 Aly/REF like target, SKAR |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC019643 |
| Gene Symbol | SKAR |
| Gene ID (NCBI) | 84271 |
| RRID | AB_2166620 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BY77 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SKAR antibody 17466-1-AP | Download protocol |
| IF protocol for SKAR antibody 17466-1-AP | Download protocol |
| IP protocol for SKAR antibody 17466-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget PDIP46 (DNA polymerase δ interacting protein 46) is an activating factor for human DNA polymerase δ. | ||
Sci Rep TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins. | ||
Am J Cancer Res Inhibition of mTORC1 signaling sensitizes hepatocellular carcinoma cells to glycolytic stress. |
