验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, pig adipose tissue, Jurkat cells, K-562 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| ChIP | See 1 publications below |
产品信息
60193-1-Ig targets PPARD in WB, ChIP, ELISA applications and shows reactivity with human, pig samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, ChIP |
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Immunogen | PPARD fusion protein Ag16770 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | peroxisome proliferator-activated receptor delta |
| Synonyms | PPAR delta, PPARB, PPAR beta, Peroxisome proliferator-activated receptor delta, Peroxisome proliferator-activated receptor beta |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC002715 |
| Gene Symbol | PPARD |
| Gene ID (NCBI) | 5467 |
| RRID | AB_10896827 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q03181 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Peroxisome proliferator-activator receptor delta(PPARD), a ligand-activated transcription factor, belongs to the PPARs family, which get its name for their chemicals that induce proliferation of peroxisomes, organelles that contributes to the oxidation of fatty acids. PPARD is a receptor for peroxisome proliferator and once activated by a ligand, it binds to and activates the promoter elements of target genes, such as acylCoA oxidase gene. But it act as a repressor for the NPC1L1 gene.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPARD antibody 60193-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Liver Int PPARβ/δ activation protects against hepatic ischaemia-reperfusion injury
| ||
Am J Physiol Endocrinol Metab Zinc finger protein 407 overexpression upregulates PPAR target gene expression and improves glucose homeostasis in mice | ||
Front Aging Neurosci A tau fragment links depressive-like behaviors and cognitive declines in Alzheimer's disease mouse models through attenuating mitochondrial function | ||
J Ethnopharmacol Anchang Yuyang Decoction inhibits experimental colitis-related carcinogenesis by regulating PPAR signaling pathway and affecting metabolic homeostasis of host and microbiota | ||
Adv Sci (Weinh) The Loss of an Orphan Nuclear Receptor NR2E3 Augments Wnt/β-catenin Signaling via Epigenetic Dysregulation that Enhances Sp1-β catenin-p300 Interactions in Hepatocellular Carcinoma |