PPID Polyclonal antibody
PPID Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, A549 cells, human brain tissue, Jurkat cells, MCF-7 cells, NIH/3T3 cells, Raji cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse testis tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy PPID Ready-To-Use IHC Kit. PPID primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12716-1-AP targets PPID in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IF, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bovine |
| Immunogen | PPID fusion protein Ag3409 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | peptidylprolyl isomerase D |
| Synonyms | CYP40, Cyclophilin-related protein, Cyclophilin-40, Cyclophilin D, Cyclophilin 40 |
| Calculated Molecular Weight | 370 aa, 41 kDa |
| Observed Molecular Weight | 38-41 kDa |
| GenBank Accession Number | BC030707 |
| Gene Symbol | PPID |
| Gene ID (NCBI) | 5481 |
| RRID | AB_2237551 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08752 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPID antibody 12716-1-AP | Download protocol |
| IHC protocol for PPID antibody 12716-1-AP | Download protocol |
| IF protocol for PPID antibody 12716-1-AP | Download protocol |
| IP protocol for PPID antibody 12716-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cardiovasc Toxicol PM2.5-Induced Programmed Myocardial Cell Death via mPTP Opening Results in Deteriorated Cardiac Function in HFpEF Mice. | ||
Mol Med Rep Downregulation of microRNA‑199a‑5p attenuates hypoxia/reoxygenation‑induced cytotoxicity in cardiomyocytes by targeting the HIF‑1α‑GSK3β‑mPTP axis. | ||
Eur J Pharmacol A novel ent-kaurane diterpenoid analog, DN3, selectively kills human gastric cancer cells via acting directly on mitochondria. | ||
Dev Cell Deregulation of ER-mitochondria contact formation and mitochondrial calcium homeostasis mediated by VDAC in fragile X syndrome | ||
Bone Res Sensory nerves directly promote osteoclastogenesis by secreting peptidyl-prolyl cis-trans isomerase D (Cyp40) | ||
J Hazard Mater T-2 toxin induces mitochondrial dysfunction in chondrocytes via the p53-cyclophilin D pathway |
