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验证数据展示

  • WB analysis of human liver using 12985-1-AP

    WB analysis of human liver using 12985-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12985-1-AP

    IHC staining of human prostate cancer using 12985-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12985-1-AP (PPIG antibody) at dilution of 1:100 (under 10x lens).

  • IF Staining of HeLa using 12985-1-AP

    IF Staining of HeLa using 12985-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12985-1-AP (PPIG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PPIG Polyclonal antibody

PPIG Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12985-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Cyclophilin G, Clk-associating RS-cyclophilin, CASP10, CARS-Cyp, CARS-cyclophilin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman liver tissue, Jurkat cells, NIH/3T3 cells, SH-SY5Y cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

12985-1-AP targets PPIG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse
文献引用反应性human
免疫原 PPIG fusion protein Ag3622 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidylprolyl isomerase G (cyclophilin G)
别名 Cyclophilin G, Clk-associating RS-cyclophilin, CASP10, CARS-Cyp, CARS-cyclophilin
计算分子量 739 aa, 87 kDa
观测分子量 89 kDa
GenBank蛋白编号BC001555
基因名称 PPIG
Gene ID (NCBI) 9360
RRIDAB_2169281
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13427
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPIG antibody 12985-1-APDownload protocol
IHC protocol for PPIG antibody 12985-1-APDownload protocol
IF protocol for PPIG antibody 12985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Sci Adv

High-throughput tagging of endogenous loci for rapid characterization of protein function

Authors - Joonwon Kim
View Article