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PPIL3 Polyclonal antibody

PPIL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15671-1-AP

Print datasheet

Synonyms

Cyclophilin J, Cyclophilin like protein PPIL3, CYPJ, PPIase, PPIL3, Rotamase PPIL3

验证数据展示

  • WB analysis using 15671-1-AP

    WB analysis using 15671-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15671-1-AP (PPIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15671-1-AP

    IHC staining of human kidney using 15671-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15671-1-AP (PPIL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPIL3 antibody included
  • IHC staining of human kidney using 15671-1-AP

    IHC staining of human kidney using 15671-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15671-1-AP (PPIL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPIL3 antibody included

经过测试的应用

Positive WB detected inHCT 116 cells, mouse brain tissue, mouse liver tissue
Positive IHC detected inhuman kidney tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PPIL3 Ready-To-Use IHC Kit. PPIL3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

15671-1-AP targets PPIL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen PPIL3 fusion protein Ag8137 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptidylprolyl isomerase (cyclophilin)-like 3
Synonyms Cyclophilin J, Cyclophilin like protein PPIL3, CYPJ, PPIase, PPIL3, Rotamase PPIL3
Calculated Molecular Weight 161 aa, 18 kDa
GenBank Accession NumberBC007693
Gene Symbol PPIL3
Gene ID (NCBI) 53938
RRIDAB_2169615
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2H8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPIL3 antibody 15671-1-APDownload protocol
IHC protocol for PPIL3 antibody 15671-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIHC

BMC Pregnancy Childbirth

Core biomarkers analysis benefit for diagnosis on human intrahepatic cholestasis of pregnancy

Authors - Yan Fang
View Article