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验证数据展示

  • WB analysis of PC-3 using 18801-1-AP

    WB analysis of PC-3 using 18801-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18801-1-AP (PPP1R9A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 18801-1-AP

    IF Staining of HepG2 using 18801-1-AP

    Immunofluorescent analysis of HepG2 cells, using PPP1R9A antibody 18801-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

PPP1R9A Polyclonal antibody

PPP1R9A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 18801-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA1222, Neurabin 1, Neurabin I, NRB1, NRBI, PPP1R9A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells
Positive IF/ICC detected inHepG2 cells, Hela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18801-1-AP targets PPP1R9A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 9A
Synonyms KIAA1222, Neurabin 1, Neurabin I, NRB1, NRBI, PPP1R9A
Calculated Molecular Weight 123 kDa
Observed Molecular Weight 180 kDa
GenBank Accession NumberBC150636
Gene Symbol PPP1R9A
Gene ID (NCBI) 55607
RRIDAB_10597849
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULJ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1R9A, also named as KIAA1222 and NEB1, binds to actin filaments (F-actin) and shows cross-linking activity. PPP1R9A has been found to be concentrated in the synapse of developed neurons and in the lamellipodia of the growth cone during neuron development.PPP1R9A may be involved in neurite formation. PPP1R9A inhibits protein phosphatase 1-alpha activity.

实验方案

Product Specific Protocols
WB protocol for PPP1R9A antibody 18801-1-APDownload protocol
IF protocol for PPP1R9A antibody 18801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols