验证数据展示
经过测试的应用
Positive WB detected in | PC-3 cells |
Positive IF/ICC detected in | HepG2 cells, Hela cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
18801-1-AP targets PPP1R9A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 9A |
Synonyms | KIAA1222, Neurabin 1, Neurabin I, NRB1, NRBI, PPP1R9A |
Calculated Molecular Weight | 123 kDa |
Observed Molecular Weight | 180 kDa |
GenBank Accession Number | BC150636 |
Gene Symbol | PPP1R9A |
Gene ID (NCBI) | 55607 |
RRID | AB_10597849 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9ULJ8 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPP1R9A, also named as KIAA1222 and NEB1, binds to actin filaments (F-actin) and shows cross-linking activity. PPP1R9A has been found to be concentrated in the synapse of developed neurons and in the lamellipodia of the growth cone during neuron development.PPP1R9A may be involved in neurite formation. PPP1R9A inhibits protein phosphatase 1-alpha activity.
实验方案
Product Specific Protocols | |
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WB protocol for PPP1R9A antibody 18801-1-AP | Download protocol |
IF protocol for PPP1R9A antibody 18801-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |