验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HEK-293 cells, HeLa cells, K-562 cells, Raji cells, SH-SY5Y cells, C6 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15617-1-AP targets PRC1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP, IF |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PRC1 fusion protein Ag8028 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein regulator of cytokinesis 1 |
| 别名 | ASE1 |
| 计算分子量 | 72 kDa |
| 观测分子量 | 66 kDa |
| GenBank蛋白编号 | BC003138 |
| 基因名称 | PRC1 |
| Gene ID (NCBI) | 9055 |
| RRID | AB_2169527 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43663 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRC1 antibody 15617-1-AP | Download protocol |
| IHC protocol for PRC1 antibody 15617-1-AP | Download protocol |
| IF protocol for PRC1 antibody 15617-1-AP | Download protocol |
| IP protocol for PRC1 antibody 15617-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Therapeutic targeting of the PLK1-PRC1-axis triggers cell death in genomically silent childhood cancer. | ||
Dev Cell Distinct classes of lagging chromosome underpin age-related oocyte aneuploidy in mouse. | ||
Biomaterials Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis. | ||
PLoS Biol The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization. | ||
Eur J Med Chem Identification of naphthalimide-derivatives as novel PBD-targeted polo-like kinase 1 inhibitors with efficacy in drug-resistant lung cancer cells | ||
Development Aurora kinase B inhibits aurora kinase A to control maternal mRNA translation in mouse oocytes. |