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- KD/KO Validated
PRC1 Polyclonal antibody
PRC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, HEK-293 cells, HeLa cells, K-562 cells, Raji cells, SH-SY5Y cells, C6 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15617-1-AP targets PRC1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, CoIP, IF |
Tested Reactivity | human, rat |
Cited Reactivity | human, mouse |
Immunogen | PRC1 fusion protein Ag8028 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | protein regulator of cytokinesis 1 |
Synonyms | ASE1 |
Calculated Molecular Weight | 72 kDa |
Observed Molecular Weight | 66 kDa |
GenBank Accession Number | BC003138 |
Gene Symbol | PRC1 |
Gene ID (NCBI) | 9055 |
RRID | AB_2169527 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O43663 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PRC1 antibody 15617-1-AP | Download protocol |
IHC protocol for PRC1 antibody 15617-1-AP | Download protocol |
IF protocol for PRC1 antibody 15617-1-AP | Download protocol |
IP protocol for PRC1 antibody 15617-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Therapeutic targeting of the PLK1-PRC1-axis triggers cell death in genomically silent childhood cancer. | ||
Dev Cell Distinct classes of lagging chromosome underpin age-related oocyte aneuploidy in mouse. | ||
Biomaterials Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis. | ||
PLoS Biol The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization. | ||
Eur J Med Chem Identification of naphthalimide-derivatives as novel PBD-targeted polo-like kinase 1 inhibitors with efficacy in drug-resistant lung cancer cells | ||
Development Aurora kinase B inhibits aurora kinase A to control maternal mRNA translation in mouse oocytes. |